Sequencing of Large Protein Molecules Using Mass Spectrometry (MS/MS)
Sensitive and Fast Analysis Identification of Unknown, Blocked (Compared to Edman Degradation) or Modified Proteins
Peptide Separation and Identification:
Proteins are digested with an enzyme to produce peptides (Why peptides? The solubility, sensitivity and MS accuracy of a peptide is much higher than a protein)
Peptides charged, separated, fragmented and identified (MS/MS breaks the peptides into fragment ions and then measures the mass-to-charge ratio of each fragment ion)
MS and MS/MS of peptides enables high throughput identification and characterization of large protein molecules.